Basics of using ImageJ for biological image analysis.
Basics of using ImageJ for biological image analysis. Topics covered: Multi-part TIFF file • Stitch together image stacks • RAM • Add labels and annotations • 8 bit, numbering conventions Minutewise stack • Measuring length, area, point manager • Color profile tool GFP and Phase, part 1 • Stack to images • Sub-stacks • Thresholds • Binary: fill holes, create ROIs, measure • Merge channels GFP and Phase, part 2 • Auto balance, individual vs. stack • Rolling ball algorithm: erase background • Donuts: expand or dilate Credit to Livio Dukaj, a grad student who taught me much of how to use this. Also credit to Dr. Nicholas Rhind at UMass Medical School, who gave me permission to use this data in my demonstration.
Basics of using ImageJ for biological image analysis. Topics covered: Multi-part TIFF file • Stitch together image stacks • RAM • Add labels and annotations • 8 bit, numbering conventions Minutewise stack • Measuring length, area, point manager • Color profile tool GFP and Phase, part 1 • Stack to images • Sub-stacks • Thresholds • Binary: fill holes, create ROIs, measure • Merge channels GFP and Phase, part 2 • Auto balance, individual vs. stack • Rolling ball algorithm: erase background • Donuts: expand or dilate Credit to Livio Dukaj, a grad student who taught me much of how to use this. Also credit to Dr. Nicholas Rhind at UMass Medical School, who gave me permission to use this data in my demonstration.